Team: Fundamental and clinical pharmacology of Pain (http://www. https://neurodol.uca.fr)
Location: Laboratoire de Pharmacologie Fondamentale et Clinique de la Douleur, Neuro-Dol (UMR Inserm 1107), Clermont-Ferrand, France
Start date: September 2021
We seek to hire a highly motivated postdoctoral researcher to 1/ characterize HCN activity in brain areas involved in pain processing and to 2/ investigate the efficacy of a new pharmacological strategy of HCN modulation to reduce chronic pain and comorbidities (anxiety and depression) in a mice model of chemotherapy-induced peripheral neuropathy, using a combination of approaches including brain slice electrophysiology, cell imaging, stereotactic brain injections and genetically-modified mice.
The candidate should hold a PhD in Neurobiology or Neuropharmacology and have a solid background in cellular electrophysiology (patch-clamp in brain slices). The candidate should also show strong interest in pain research and molecular/cellular neuroscience with special focus on ion channels pharmacology. A background in molecular biology and pain beahavior assessment would be a plus.
The project is part of a recently awarded collaborative research project-ANR Grant developed and coordinated by Dr. Jérôme Busserolles, in a team recognized internationally for its work in the pain field, and in collaboration with teams with long-standing expertise in pain (IGF-UMR 5203 in Montpellier) as well as in drug design field (ICCF-UMR 6296 in Clermont-Ferrand). The position has 2 years of funding.
The candidate will benefit of a stimulating, interactive and collaborative research environment. The team is part of the Neuro-dol UMR Inserm 1107 lab, dedicated to pain research, and has an overall focus on studying cellular and molecular mechanisms underlying chronic pain and affective/cognitive comorbidities and on developing new pharmacological therapeuthic strategies.
Post-doctoral position in the modulation of GPCR phramacology with nanobodies Physiology of Reproduction and Behaviours Unit, Nouzilly, France.
The project in which the post-doctoral fellow will be involved is based on the discovery, characterization and optimization of nanobodies capable of selectively modulating the activity of GPCRs controlling follicular development, steroidogenesis and ovulation.
Qualifications: The post-doctoral fellow will have to perform: i) pharmacological profiling and trafficking measurements using HTRF and BRET methods; ii) phage-display; iii) production, purification and characterization of recombinant proteins; iv) kinetic-rate constants determination; v) flow cytometry; vi) ELISA/HTRF assays; vi) histology/immunohistochemistry. The successful candidate will have prior experience in several of these approaches. He/she must have worked and/or have solid knowledge in biochemistry, cell biology, molecular pharmacology. Experience/knowledge in reproductive biology, antibody development/engineering, GPCR pharmacology and/or in vivo testing/phenotyping will also be appreciated. We seek for a highly motivated candidate capable of working both independently and as part of a team; endowed of excellent problem solving, organizational and communication skills. Prior international experience will be considered as an asset.
Lab website: http://bios.tours.inra.fr/
Junior R&D Project Leader in Biochemistry at CALIXAR (CDI - CALIXAR- Lyon, France)
You will participate in the development and the successful realization of R&D challenging projects for different clients, pharmaceutical and biotech companies (USA, Europe, Japan, ...). In contact with these clients and under the supervision of your project leader, you will propose and implement co-development programs in line with their specifications all the way through to the completion of the projects. With driving force, you will be involved in implementing the best state of the art in the field, researching and developing the new protocols. You will carry out the implementation of the different techniques (expression, extraction, purification, stabilization, characterization) for the generation and study of complex targets (especially membrane proteins) in the laboratory and/or with the help of partners. You will report on project results and follow-up to your clients and could be involved in drafting patents and publications. Finally, you will ensure a regular scientific watch allowing you to establish the technical expertise of the company.
The candidate will be in full autonomy as soon as possible on client projects.
A permanent INRAE researcher position in artificial intelligence, statistics and cell signaling networks is open for 2021 external competitions in the BIOS (Biologie des Systèmes de Signalisation) team within the PRC (Physiologie de la Reproduction et des Comportements) unit in Nouzilly near Tours (37).
See the research profil on INRAE Jobs site: https://jobs.inrae.fr/en/open-competitions/open-competions-research-scientists-job-profiles-crcn/cr-2021-phase-2
Application deadline: March 04, 2021.
POSTDOCTORAL POSITION IN GPCR PHARMACOLOGY
Institut des Biomolécules Max Mousseron (IBMM), Montpellier
Working environment: A 18 months postdoctoral position is available starting on March 1st 2021 in the “Cell Pharmacology” group of the Institut des Biomolécules Max Mousseron (IBMM –UMR CNRS 5247). The IBMM is a multidisciplinary research center that provides a stimulating and collaborative working environment for scientists conducting research on the design, the synthesis and the pharmacology of potential future drugs.Our group is interested in how the signal is transmitted via a particular class of membrane receptors, G protein-coupled receptors (GPCRs). The project in which the post-doctoral fellow will be involved is based on the characterization of the complex formed by the ghrelin and dopamine receptors; this heteromer is directly involved in the control of food intake.
Main mission: The post-doctoral fellow will be in charge of the characterization of bivalent compounds targeting the dimer formed by the ghrelin and dopamine receptors using model systems of increasing complexity (purified receptors, cell lines, animal models), to both validate this complex as a possible target for treating food-intake disorders and illuminate the impact of receptor dimerization on ghrelin-mediated signaling.
Activities: The post-doctoral fellow will have to delineate the pharmacological profiles of the ligands-binding affinity, activation of G proteins, production of second messengers, recruitment of arrestin using the receptors purified in lipid nanodiscs as well as cell lines expressing the different combinations of receptors. As part of his/her activity, he/she will have to design the experimental plan, implement the corresponding studies, analyze the results, put them back in the context of the literature and communicate them through oral and written presentations.
Qualifications: The post-doctoral fellow will have to perform ligandbinding and signaling protein activation experiments using fluorescence and bioluminescence transfer methods, essentially. The candidate will thus need to have an experience in a related topic. He/she must have worked and/or have solid knowledge in biochemistry (protein handling), cell biology (cell culture, transfection), pharmacology (binding assays, production of second messengers, etc.) as well as basic knowledge of medicinal chemistry concepts. Experience and/or knowledge in fluorescence and bioluminescence transfer methods (HTRF, BRET, FRET) will also be appreciated, as will expertise in GPCR pharmacology. The candidate is expected to be able to work both independently and as part of a team and he/she will have with excellent problem-solving, organizational and communication skills.
Lab website:https://www.ibmmpharmaco.com/More information on salary and conditions at: https://euraxess.ec.europa.eu/jobs/584097